Fig. 8

Effects of heat-killed P. intermedia on the transcriptomics of GABA signaling and GABAergic synapses in transplanted tumor model mice. a Top 20 downregulated pathways between the heat-killed P. intermedia and control groups in KEGG pathway enrichment analysis. Red triangles indicate the GABA-related signaling pathway. b–g GSEA showing the downregulation in the heat-killed P. intermedia group. b GABAergic synapse downregulation. c Glutamatergic synapse downregulation. d GABA signaling pathway downregulation. e Neuroactive ligand‒receptor interaction downregulation. f Synaptic vesicle cycle downregulation. g GABA synthesis, release, reuptake and degradation downregulation. h Heatmap of the GABA synthesis, release, reuptake and degradation gene set. i Heatmap of the GABA signaling pathway gene set. Red asterisks indicate important genes in the GABA-related signaling pathway. j Representative images of GABBR2 IHC staining. The relative AOD of GABBR2 IHC staining was compared between the control and heat-killed P. intermedia groups. The data are shown as the mean ± SD and were analyzed by t test. ***P < 0.001