Fig. 6

ADAR1 deficiency simultaneously heightens the effects of tBHP on intracellular ROS accumulation and regulation of Keap1/Nrf2 pathway in HCC cells. A–C DCFH-DA fluorescent probe for measuring intracellular ROS level of HepG2 and SMMC-7721 cells with tBHP administration and/or sh-ADAR1 transfection. Bar, 20 μm. D–H Immunoblot analysis of Keap1 or Nrf2 expression in HepG2 and SMMC-7721 cells with tBHP administration and/or sh-ADAR1 transfection. I Co-IP assay of Keap1 and Nrf2 in ADAR1 precipitates in HepG2 and SMMC-7721 cells with tBHP administration and/or sh-ADAR1 transfection. J Multiple immunofluorescence images of ADAR1 and Keap1 in HepG2 and SMMC-7721 cells with tBHP administration and/or sh-ADAR1 transfection. Bar, 10 μm. K Multiple immunofluorescence images of ADAR1 and Nrf2 in HepG2 and SMMC-7721 cells with tBHP administration and/or sh-ADAR1 transfection. Bar, 10 μm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001