Fig. 4

ADAR1 mediates Keap1/Nrf2 pathway in HCC. A–F Immunohistochemistry of Ki-67, Caspase-3, ADAR1, Keap1, and Nrf2 expression in mouse tumors with sh-NC or sh-ADAR1. Bar, 50 μm. G–P Immunoblot analysis of Keap1 or Nrf2 expression in HepG2 and SMMC-7721 cells with sh-ADAR1 or ADAR1 overexpression transfection. Q Co-IP assay of Keap1, and Nrf2 in ADAR1 precipitates in HepG2 and SMMC-7721 cells with sh-NC or sh-ADAR1. R Multiple immunofluorescence images of ADAR1 and Keap1 in tumor tissues from sh-NC or sh-ADAR1 mice. Bar, 10 μm. S Multiple immunofluorescence images of ADAR1 and Nrf2 in tumor tissues from sh-NC or sh-ADAR1 mice. Bar, 10 μm. **p < 0.01; ***p < 0.001; ****p < 0.0001