Fig. 2

ADAR1 loss attenuates proliferative capacity of HCC cells. A, B Real-time quantitative PCR of ADAR1 expression in HepG2 and SMMC-7721 cells transfected with sh-NC or three shRNAs against ADAR1. C–F Immunoblot analysis of ADAR1 expression in HepG2 and SMMC-7721 cells transfected with sh-NC or three shRNAs against ADAR1. G, H Real-time quantitative PCR of ADAR1 expression in HepG2 and SMMC-7721 cells with ADAR1 overexpression transfection. I–L CCK-8 for cell viability of HepG2 and SMMC-7721 cells with sh-ADAR1 or ADAR1 overexpression transfection. M–P EdU staining for proliferation of HepG2 and SMMC-7721 cells with sh-ADAR1 or ADAR1 overexpression transfection. Bar, 20 μm. Q Representative images of BALB/c nude mice at day 28 after inoculating sh-NC or sh-ADAR1 luciferase-tagged SMMC-7721 cells. R, S Tumor weight and tumor growth curve. Ns: no significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001