Fig. 2

Inherited susceptibility to leukemia in Gata1.05/X mice (G1^1.05) in 129X1/SvJ and C57BL/6J backgrounds. A Schematic illustration of mouse mating strategy. N6 or deeper generation C.G1^1.05 mice were prepared by backcrossing G1^1.05 in a mixed background with BALB/c mice six and more generations. These C.G1^1.05 were crossed with 129X1/SvJ wild-type males, and subsequently F1-C:129.G1^1.05 (shown in gray) were generated. Note that F1-C:129.G1^1.05 display heterozygosity at the Gata1 locus (i.e., Gata1.05 and wild-type alleles), with equal contributions from the BALB/c and 129X1/SvJ backgrounds on all autosomes. G1^1.05 generated by crossing F1-C:129.G1^1.05 with wild-type 129X1/SvJ males are defined as N2-129.G1^1.05 (shown in blue). We also generated F2-C:129.G1^1.05 (shown in purple) through brother-sister crossing of F1-C:129.G1^1.05 and F1-C:129 males. Thus, N2-129.G1^1.05 (blue) harbor either a homozygous 129X1/SvJ background or a heterozygous BALB/c:129X1/SvJ background. Similarly, F2-C:129.G1^1.05 (purple) harbor a homozygous BALB/c background, a homozygous 129X1/SvJ background, or a heterozygous BALB/c:129X1/SvJ background. Details of the mice examined are provided in Supplementary Table 2B Schematic illustration of mouse mating strategy. N6 or deeper generation C.G1^1.05 mice were used. G1^1.05 obtained by crossing C.G1^1.05 with wild-type C57BL/6J males are defined as F1-C:B6.G1^1.05 (shown in turquois). Crossing F1-C:B6.G1^1.05 with wild-type BALB/c and C57BL/6J males generated N2-C.G1^1.05 (shown in red) and N2-B6.G1^1.05 (shown in black), respectively. Note that F1-C:B6.G1^1.05 display heterozygosity at the Gata1 locus (i.e., Gata1.05 and wild-type alleles), with equal contributions from the BALB/c and C57BL/6J backgrounds on all autosomes. N2-C.G1^1.05 (red) harbor a BALB/c:C57BL/6J heterozygous background or a BALB/c homozygous background, and N2-B6.G1^1.05 (black) harbor a BALB/c:C57BL/6J heterozygous background or a C57BL/6J homozygous background. Details of the mice examined are provided in Supplementary Table 3C Kaplan-Meier curves of the overall survival of F1 C:129.G1^1.05 (gray line, n = 35), N2 129.G1^1.05 (blue line, n = 9), and F2C:129.G1^1.05 (purple line, n = 156). D Kaplan-Meier curves of the overall survival of F1-C:B6.G1^1.05 (turquois line, n = 30), N2-C.G1^1.05 (red line, n = 57), and N2-B6.G1^1.05 (black line, n = 264). Results of Log-rank tests and Generalized Wilcoxson tests in comparison of N2-B6.G1^1.05 vs. F1-B6:C.G1^1.05 and N2-B6.G1^1.05 vs. N2-C.G1^1.05 are indicated. E Observed mortality data for mice used in C and necropsy results. F Observed mortality data for mice used in D and necropsy results