Fig. 4

DAC enhances RSL3-induced ferroptosis by regulating the AMPK-SLC7A11-GPX4 signaling pathway. (A) AMPK, p-AMPK, SLC7A11, and GPX4 protein levels were measured in MOLM-13 and MV4-11 cells treated with DAC (0.5 µM) for 48 h, RSL3 (0.05 µM) for 24 h, or their combination DAC (0.5 µM) + RSL3 (0.05 µM) (D + R). (B and C) AMPK transcript level (B) and GPX4 enzyme activity (C) were measured in MOLM-13 and MV4-11 cells treated with Ctrl, DAC, RSL3, or D + R cotreatment. (D and E) AMPK and SLC7A11 transcripts were measured in MOLM-13 and MV4-11 cells transduced with shRNAs for AMPK (sh-AM#1 and #2) or negative control (sh-NC). (F) AMPK, p-AMPK, SLC7A11, and GPX4 protein levels were measured in MOLM-13 and MV4-11 cells transduced with sh-AM or sh-NC. (G and H) Viability was measured in MOLM-13 and MV4-11 cells transduced with sh-AM#2 or sh-NC, which were further treated with RSL3 (0.05 µM) or Ctrl for 24 h. (I and J) Lipid ROS levels were measured in MOLM-13 and MV4-11 cells transduced with sh-AM#2 or sh-NC, which were further treated with RSL3 or Ctrl for 24 h. **P < 0.01; ***P < 0.001. N.S: not significant