Fig. 5

LAMP5-AS1- LAMP5 axis regulate cell autophagy. (a, b) Representative IF graphs (left) showing LC3B puncta accumulation in THP1(a) and MV4-11(b) cells. Histogram plots show the statistical values for the number of LC3B puncta per cell calculated by Image-Pro Plus (right, n = 20 cells; Error bars reflect ± SEM, ***, P < 0.001). Scale bar, 10 mm. (c) Representative TEM graphs (left) showing autolysosomes (indicated by arrows) in THP1 cells. Autolysosomes accumulated in the LAMP5-AS1- or LAMP5-downregulated cells than those in si-NC samples. Error bars reflect ± SEM (ten cells per group, ***, p < 0.001). (d) Western blotting showing LC3B-II enrichment after LAMP5-AS1 or LAMP5 knockdown in THP1 and in MV4-11 cells. beta-tubulin as the internal control. The LC3B-II /Tubulin densitometric ratio were recorded by ImageJ