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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Blockade of the lncRNA-DOT1L-LAMP5 axis enhances autophagy and promotes degradation of MLL fusion proteins

Fig. 3

LAMP5 expression level is regulated by LAMP5-AS1. (a) Schematic depiction of LAMP5-AS1 and LAMP5 gene loci. (b, c) The co-expression of LAMP5-AS1 and LAMP5 was validated in leukemia patient samples (b) and leukemia cells(c). Spearman method was used to analyze the correlation coefficient. (d, e) Quantitative RT-PCR and western blotting show that Knocking down LAMP5-AS1 by siRNAs reduce LAMP5 expression in MLL leukemia cell lines (d) and primary cells (e). Error bars reflect ± SEM (*, p < 0.05; **, p < 0.01; ***, p < 0.001) from three independent experiments. (f) Lentiviral overexpression of LAMP5-AS1 increased LAMP5 expression in MLL leukemia cells. Error bars reflect ± SEM (*, p < 0.05; **, p < 0.01; ***, p < 0.001) from three independent experiments. The LAMP5/ Tubulin densitometric ratio was recorded by ImageJ

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Experimental Hematology & Oncology

ISSN: 2162-3619

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