Fig. 1

Targeting LAMP5-AS1 induces MLL leukemia cell apoptosis and decreases MLL fusion protein. (a, b) Cell apoptosis was measured by flow cytometry in both MLL leukemia cell lines (MV4-11, MOLM-13 and THP1) (a) and two primary cells from patients with MLL leukemia (b) transfected with LAMP5-AS1 siRNA and the control. Error bars reflect ± SEM (**, p < 0.01, ***, p < 0.001, right) in three independent experiments. (c) Quantitative RT-PCR for MLL-AF4 (MV4-11) and MLL-AF9 (THP1) under knockdown of LAMP5-AS1. NS: not significant. (d, e) Western blotting was performed to detect MLL fusion protein levels in cell lines (d) and primary cells (e) when LAMP5-AS1 was knocked down. The MLL-AF9, -AF4 or -ENL/ Tubulin densitometric ratio was recorded by ImageJ