Fig. 2

Tumor B-cell copy number variation (CNV) promotes the evolution of therapeutic resistance. A Left: UMAP visualization of all cells with B cells highlighted in blue. Right: UMAP visualizations of B cells colored by clinical outcomes, patient, and sample. B Heatmap displays cellular CNV profiles (row) of each cell across chromosomes (columns) for all samples (top) and restricted to longitudinal samples (bottom). Colors reflect copy number gains (red) and losses (blue). Sample names and clinical outcomes are annotated on the left. Samples are ordered by aggressiveness from the top (normal) to the bottom (Dual-R). C Plot shows the inferred copy number estimates (y-axis) for samples across chromosome 12 (x-axis). Horizontal dashed line represents expected normal copy number. D Boxplot shows the Euclidean distance (y-axis) derived from the CNV profile-based low dimensional space across different clinical outcomes (x-axis). E Percentages of complex karyotype in each clinical outcome group