Fig. 7
METTL1 knockdown promotes tsRNA biogenesis. A tRNA degradation levels on PAGE gels under RNase A/T1 in THP-1 cells upon METTL1 knockdown. B High-throughput small RNA sequencing analysis of tsRNA expression in THP-1 cells upon METTL1 knockdown. C Representative bands of tsRNA levels examined by RT-PCR in THP-1 cells upon METTL1 knockdown. D, E Northern blot analysis of 3′tsRNALeuCAG and 3′tsRNALeuTAA in THP-1 cells upon METTL1 knockdown. Left: NB images. Right: quantification data. F High-throughput small RNA sequencing analysis of tsRNAs expression in THP-1 cells upon METTL1 overexpression. G Representative bands of tsRNAs levels examined by RT-PCR in THP-1 cells upon METTL1 overexpression. shNC: METTL1 control, shM1: METTL1 knockdown, CON: METTL1 control, OE-M1: METTL1 overexpression. Data were presented as mean ± SEM (Student’s t test, ****p < 0.0001)