Fig. 6

METTL1 knockdown leads to tRNA dysregulation for nascent protein synthesis inhibition. A–D Representative tRNAs level examined by northern blot in METTL1 knockdown (shM1) and METTL1 control (shNC) THP-1 cells. Left: NB images. Right: quantification data. E Experimental procedures for OPP labeling experiments in F, G. F, G The level of OPP means fluorescence intensity (MFI) in THP-1 cells transfected with tRNAs extracted from METTL1 knockdown (shM1) or METTL1 control (shNC) THP-1 cells, respectively. F Quantification data. G Representative images of flow cytometry analysis. Data were presented as mean ± SEM (Student’s t test, ***p < 0.001, ****p < 0.0001)