Fig. 4
Knockdown of METTL1 leads to a reduction in the abundance of cellular m7G modification on tRNA and mRNA in THP-1 cells. A Experimental procedures for detecting RNA modifications in THP-1 cells. B, C Radar map (B) and heatmap (C) showed the comparison of RNA modification levels in tRNA-enriched fragments (tRNA, ~ 80nt) in METTL1 knockdown and control THP-1 cells. D Comparison of m7G modification level on tRNA in METTL1 knockdown and control THP-1 cells. E, F Radar map (E) and heatmap (F) showed the comparison of RNA modification levels in mRNA in METTL1 knockdown and control THP-1 cells. G Comparison of m7G modification level on mRNA in METTL1 knockdown and control THP-1 cells. Data were presented as mean ± SEM (Student’s t test, ***p < 0.001, ****p < 0.0001)