Fig. 3

DNA methylation regulates METTL1 expression and the correlation of METTL1 and WDR4 in AML cells. A Comparison of METTL1 DNA methylation level in healthy individuals (NC) and AML patients from TCGA dataset. B Correlation between mRNA expression level (FPKM) and DNA methylation level of METTL1 in AML patients from TCGA dataset. C, D The mRNA (C) and protein (D) expression level of METTL1 in AML cells while treated with bobcat339 hydrochloride. E Correlation between METTL1 and WDR4 mRNA levels in AML patients from TCGA dataset. F Correlation between the METTL1 and WDR4 expression levels and overall survival of AML patients from TCGA dataset. G The protein expression level of WDR4 in METTL1 knockdown (shM1) and METTL1 control (shNC) AML cells. H The protein expression level of WDR4 in METTL1 over-expression (OE-M1) and METTL1 control (CON) HL60 cells. I The mRNA expression level of WDR4 in METTL1 knockdown (shM1) and METTL1 control (shNC) AML cells. J The mRNA expression level of WDR4 in METTL1 over-expression (OE-M1) and METTL1 control (CON) HL60 cells. K, L The protein (K) and mRNA (L) expression level of METTL1 in WDR4 over-expression (OE-WDR4) and WDR4 control (CON) HL60 cells. Data were presented as mean ± SEM (Student’s t test, *p < 0.05, **p < 0.01, ****p < 0.0001)