Fig. 8

ZMIZ2 activated the Wnt/β-catenin signaling pathways through LEF1. (A) Heat map shows the top 15 variated Hallmark pathways between ZMIZ2 expression subgroups by GSVA. (B) KEGG analysis for DEGs screened by transcriptome sequencing. (C) Heat map shows expressions of the DEGs associated with Wnt/β-catenin signaling pathway between siZMIZ2 and control group. (D) The mRNA levels of 10 Wnt/β-catenin pathway related genes with ZMIZ2 knockdown and overexpression were detected by qRT-PCR. (E) Expression level of LEF1 in TCGA-LIHC cohort and GSE55092 dataset and correlation of ZMIZ2 expression with LEF1 expression. (F) LEF1 and Wnt/β-catenin pathway expression was regulated by ZMIZ2. (G) The interaction between ZMIZ2 and LEF1 in HCC cells was investigated using exogenous immunoprecipitation (IP) assay. *p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001; ns, not significant