Fig. 8

Impact of combining FAK inhibitor and PD-1 blockade on TME in KL mouse model. A Representative IHC staining of collagen I and quantification of collagen I staining area in different treatment schedule shown in Fig. 7A). Scale bars, 200 μm. B Representative IF staining of α-SMA and quantification of α-SMA staining area in different treatment schedule shown in Fig. 7A). Scale bars, 100 μm. C Representative IF staining of FAP and quantification of FAP staining area in different treatment schedule shown in Fig. 7A). Scale bars, 100 μm. D Quantification of CD8⁺TILs and ratio of CD8⁺T cells to Treg in each group. E Representative CD8 IHC staining and quantification of intratumoral primary tumor regions in each group. Scale bars, 100 μm. F Representative CD8 IHC staining and quantification of metastatic lung tumor regions in each group. Scale bars, 100 μm. Inset scale bars, 50 μm. Unpaired Student’s t-test was performed and results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ^nsp-values with no statistical difference. FAK, focal adhesion kinase; PD-1, programmed death-1; TME, tumor microenvironment; IHC, immunohistochemistry; IF, immunofluorescence; FAP, fibroblast activation protein; TIL, tumor infiltrating lymphocyte; Treg, regulatory T cell; SEM, standard error of mean