Fig. 7
Combining FAK inhibitor and PD-1 blockade reduced tumor growth and lung metastasis. A Schematic diagram depicting treatment schedule for KL tumors. Red bar indicating the FAK inhibitor was daily administered. Blue bar indicating PD-1 blockade was administered every 3 days for 6 doses in total. Mouse were sacrificed for tumor analysis at indicated time. B IHC staining for p-FAK in each group. Scale bars, 200 μm. C Volume change in each group with different treatment schedule (n = 5–8 per group). Two-way analysis of variance (ANOVA) was performed. D Kaplan–Meier survival curves of the subcutaneous tumor-bearing mice treated as indicated since the day of tumor volume reached 100–150 mm3. Survival time of mice was defined from the day of tumor cell inoculation until the tumor volume reached 1000 mm3(n = 5–8 per group). E HE-stained normal lungs of C57 mice and lungs from KL tumor-bearing mice at Day30. Scale bars, 50 μm. F Representative images and quantification of invasive area in HE-stained lungs of KL tumors treated with different treatment schedules. Scale bars, 2 mm. Unpaired Student’s t-test was performed and results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and nsp-values with no statistical difference. FAK, focal adhesion kinase; PD-1, programmed death-1; IHC, immunohistochemistry; HE, hematoxylin–eosin; KL, KRASG12DLKB1−/−; SEM, standard error of mean