Fig. 2

KL tumors displayed immunosuppressive TME with fewer immune-active cells and more immunosuppressive cells infiltration. Subcutaneous KP and KL tumors were dissected for flow cytometry analysis when tumor volume reached 700–800 mm³ (n = 6 tumors in KL group, n = 7 tumors in KP group). A Representative flow cytometry images and histogram showed the percentage of CD3⁺ of CD45⁺ cells in each group. B Ratio of CD19⁺ to CD45⁺ cells. C Ratio of CD3⁻NK1.1⁺ to CD45⁺ cells. D Ratio of CD8⁺ of CD45⁺ cells. E Ratio of CD4⁺ to CD45⁺ cells. F Ratio of CD4⁺Foxp3⁺ to CD3⁺ cells. G Ratio of CD11c⁺MHCII⁺ of CD45⁺ cells. H Representative flow cytometry images and histogram showed the percentage of CD11b⁺F4/80⁻Ly6G⁺ of CD45⁺ cells. I Representative flow cytometry images and histogram showed the percentage of macrophage (CD11b⁺F4/80⁺ of CD45⁺cells), M1-like macrophage (CD86⁺) and M2-like macrophage (CD206⁺). J Gating strategy for MDSCs and histogram showed the percentage of M-MDSC (Ly6C⁺Ly6G⁻) and PMN-MDSC (Ly6G⁺Ly6C⁻). Results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ^nsp-values with no statistical difference. KL, KRAS^G12DLKB1^−/−; KP, KRAS^G12DTP53^−/−; TME, tumor microenvironment; NK, nature killer; MHC, major histocompatibility complex; Foxp3, forkhead box P3; M-MDSC, monocytic-myeloid-derived suppressor cells; PMN-MDSC, polymorphonucler-myeloid derived suppressor cells; SEM, standard error of mean