Fig. 2

MRTX1133 reduces cell proliferation and increases apoptosis mainly through the MAPK and PI3K/AKT/mTOR signaling pathways. (A) Representative 20X immunohistochemical images of tumor sections stained for Ki67 from MRTX1133-treated and control mice. (B) Quantification of the Ki67 proliferation index in treated (n = 12) and control mice (n = 12). (C, E, G) Representative 20X immunohistochemical images of tumor sections stained for cleaved caspase-3, phospho-p44/42 (pERK1/2) and phospho-S6 from MRTX1133-treated and control mice. (D, F, H) Quantification of the percentage of positive cells and intensity of staining for cleaved caspase-3, phospho-p44/42 (pERK1/2) and phospho-S6 from MRTX1133-treated (n = 12) and control mice (n = 12). Data are represented as the mean ± SEM or stacked bar graphs (orange bars are control and blue bars are treated mice).