Fig. 1
From: Single VHH-directed BCMA CAR-NK cells for multiple myeloma
BCMA-CD28-IL15-engineered NK cells showed enhanced antitumor activity compared to BCMA-CD28 CAR-NK cells. (A) Schematic diagrams of BCMA CAR-NK constructs. (B) The fold expansion curve of NK cells, BCMA-CD28-IL15 CAR-NK cells, and BCMA-CD28 CAR-NK cells. (C) BCMA CAR expression of NK cells on day 14. (D) The cytotoxic activity of BCMA-CD28-IL15 CAR-NK cells vs. BCMA-CD28 CAR-NK cells and ex vivo-expanded NK cells against MM.1S and Daudi cells using a lactate dehydrogenase release assay (n = 3; ****, P < 0.0001). The numbers of effector cells were calculated as CAR-positive cells. NK cells were used to adjust the different CAR-positive cells. (E) MM.1S and Daudi cells were cocultured with BCMA-CD28-IL15 CAR-NK cells, BCMA-CD28 CAR-NK cells, or NK cells at an E:T ratio of 1:2 for 24 h. IFN-γ, granzyme B, and IL-15 secretion in supernatants was measured by ELISA (n = 3; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001)