Fig. 1
(A) Immunohistochemical evaluation of CXCR4 / CXCR7 in SEM, CC, YST and YST + EC mixed GCT. (B) Relative expression of CXCR4, CXCR7, and CXCL12 in GCT cell lines and fibroblasts (MPAF). ACTB and GAPDH were used as housekeeping genes. (C) Flow cytometry data of CXCR7-APC stained GCT cell lines (blue) compared with unstained controls (grey). (D) XTT cell viability assays of GCT cell lines and fibroblasts (MPAF) treated with CXCR4-NDC or CXCR4-NB alone for 24–96 h. (E) LD50 values measured by XTT cell viability assays 72 h after treatment with cisplatin (CisPt, µM) or CXCR4-NDC (nM) and color-coded changes in cell cycle distribution (mitotic catastrophe = red; changes < 5% = grey) upon treatment with CXCR4-NDC (LD50 concentrations) for 72 h as compared to the CXCR4-NB alone. (F) Lollipop graph summarizing the relative number of apoptotic cells in CXCR4+ GCT cells and CXCR4− MPAF upon treatment with either CXCR4-NDC or CXCR4-NB alone (LD50 concentrations) for 72 h