Fig. 3

In vivo CRISPR modeling in tumor and immune cells. A. Tumor cells. Cas9-expressing tumor cells transduced with sgRNA library are implanted into immunocompromised or immunocompetent mice that model the absence or presence of immune surveillance. Immunocompetent mice were treated with different immunotherapies to discover genes reflecting drug-responsiveness. B. Immune cells. A pooled CRISPR sgRNA library was transduced into T cells that express Cas9 and transgenic T cell receptor (TCR) before being adoptively transferred to mouse models. Antigen-specific T cells isolated from mice are sequenced to compare their sgRNA abundance with that of pre-transferred T cells, revealing T cell response in the context of infection, inflammation, and tumor