Fig. 3

Strategies for circRNA detection. a Northern blot to identify and quantify circRNAs with a BSJ-spanning probe. b Quantification of circRNAs by reverse transcription (RT) and quantitative PCR (qPCR) assays. A pair of divergent primers are used. c Droplet digital PCR (DdPCR) is a novel technology determines the absolute quantification of a candidate circRNA using the ratio of positive to negative droplets. d NanoString Technology captured the BSJ flanking sequences by a biotinylated probe and a reporter probe loading fluorescent barcodes, followed by a high-resolution charge-coupled device (CCD) camera and digitization. e In situ hybridization used to visualize and quantify the interest circRNAs. There are two methods, which based on an oligonucleotide probe coupled to fluorescent dyes, and a sgRNA in a dCas13a-EGFP system