Fig. 2

Workflow of BSJ-based circRNA identification. a The canonical workflow of circRNA identification tools that search for the specific BSJ sequences in the sequencing data and map to genome. b circRNA enriched by rRNA deleted and RNase R treatment before library. c circRNAs fragment into short reads and BSJ detecting in RNA sequencing. d After enrichment, the circRNA pool was nicked to generate large fragments, and then the obtained circRNA pool aligns with the ONT long-read sequencing protocol. e Discovery tools identify the full-length circRNA isoforms using rolling circle amplification followed by nanopore long-read sequencing